ANALYTICAL STANDARDIZATION OF KUKKUTNAKI GUGGULU

Abstract

Herbs are natural resources for a variety on biochemical products and are used medicinally in different countries; they are the source of many potent and effective drugs. According to retrospective literary review, the combination of Kukkutnaki (Aspidium cicutarium Sw.) and purified Guggulu (Commiphora mukul Hook. Ex Stocks) was first mentioned in book Chikitsa pradeep named as Kukkutnaki Guggulu. This Anubhut herbal formulation Kukkutnaki Guggulu was prepared according to text “Guggulu kalpana”. It was documented as a herbal drug which is used for goiter, cysts, tumors, tonsilitis, abscess. The quality test for the finished product were performed in which total ash% 6.49 ±0.553, acid insoluble ash% 1.323± 0.547, water soluble extract% 35.64±0.590, alcohol soluble extract% 20.45±0.822, pH 3.81±0.041, moisture content% 9.63±0.851, were estimated. The tablets or vati of the study drug were analysed for hardness, uniformity of weight, friability and disintegration time(DT) in which hardness(Kg/cm2) 1.42±0.080, uniformity of weight % 4.267±0.163, friability% 0.566±0.232, disintegration time (min) 9.35±0.229 were estimated. HPTLC analysis of Kukkutnaki Guggulu using toluene: ethyl acetate: formic acid(7:3:1) as mobile phase showed 14 peaks for all three batches. The coinciding max Rf values were 0.10, 0.18,0.33,0.42 and 0.80. Hence, the standard parameters were developed for current formulation. So, in the current study, the analytical standard of Kukkutnaki Guggulu was developed which will be ready reference for the next researcher.